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Revista argentina de antropología biológica
versión On-line ISSN 1514-7991
Resumen
PETRIGH, Romina S y FUGASSA, Martín H. Información molecular obtenida a partir de pieles de la colección del museo Regional Fagnano, RíoGrande, Tierra del Fuego. Rev Arg Antrop Biol [online]. 2015, vol.17, n.1, pp.29-34. ISSN 1514-7991.
Molecular techniques were applied to samples from poorly preserved furs deposited in the collection of Monseñor Fagnano Regional Museum in Tierra del Fuego, Argentina, in order to identify the species from which they were made. Hairs were obtained from guanaco leather blankets (Lama guanicoe) and a puma fur (Pumaconcolor) made by Selk'nam from Santa Cruz province. Both samples have been kept in the warehouse of Monseñor Fagnano Regional Museum at the Candelaria Salesian Mission in Río Grande, Tierra del Fuego, Argentina. For DNA extraction, 5mm hair strands were digested in a PCR-compatible lysis buffer. Specific mitochondrial DNA fragments were amplified by PCR and sequenced. Nucleotide sequences were compared with those deposited in the National Center for Biotechnology Information (NCBI), USA: GenBank. The use of molecular techniques allowed us to recover sequences from poorly preserved fur samples. This can be extended to other sources of hairs and fibers of archaeological origin in the area.
Palabras clave : ancient DNA; hair; environmental archaeology.