Resumo

ROSTAGNO, Agueda  e  GHISO, Jorge. Biochemical analysis of myoglobinuria associated with rhabdomyolysis. Acta bioquím. clín. latinoam. [online]. 2013, vol.47, n.1, pp.7-15. ISSN 0325-2957.

The destruction of skeletal muscle in a condition known as rhabdomyolysis results in the release into the blood stream of large quantities of the 17 kDa myoglobin that freely filtrates through the glomeruli, often overwhelming the capacity of the proximal tubule for reabsorption. As a result, identification of myoglobin in the urine becomes an essential tool to fully complement other biochemical parameters in the diagnosis of the disease. Using a combination of protein electrophoresis in agarose gels and immunofixation with specific antibodies, direct evidence is provided for the presence of intact myoglobin in the urine of a patient with acute kidney injury due to rhabdomyolysis triggered by undesirable side effects of specific medications. The electrophoretic/immunochemical data was further corroborated by amino acid sequence, Western blot, and mass spectrometry analysis. The simple combination of electrophoresis and immunofixation protocols provides a flexible approach that can be extended to the identification of the various proteins known to be involved in overflow proteinuria.

Palavras-chave : Protein electrophoresis; Overflow proteinuria; Myoglobinuria; Rhabdomyolysis.

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