Resumo

CATTANEO, Elizabeth Renée; PELLON-MAISON, Magalí  e  GONZALEZ-BARO, María del Rosario. Role of isoform 2 of glycerol-3-phosphate acyltransferase in testicular lipid metabolism. Acta bioquím. clín. latinoam. [online]. 2013, vol.47, n.2, pp.315-325. ISSN 0325-2957.

De novo glycerolipid synthesis begins with the acylation of glycerol-3 phosphate catalyzed by glycerol-3-phosphate acyltransferase (GPAT). In mammals, at least four GPAT isoforms have been described, differing in their cell and tissue locations and sensitivity to sulfhydryl reagents. The objective of the present work was to study localization and function of GPAT2, a mitochondrial isoform that is mainly expressed in testis, using as a model CHO-K1 cells that overexpress GPAT2. Incubation of GPAT2-transfected CHO-K1 cells with arachidonoyl-CoA increased GPAT activity 2-fold and the incorporation of [1-14C] arachidonate into TAG. Consistently, arachidonic acid was present in the TAG fraction of cells that overexpressed GPAT2, but not in control cells, corroborating GPAT2's role in synthesizing TAG that is rich in arachidonic acid. In rat and mouse testis, Gpat2 mRNA was expressed only in primary spermatocytes; the protein was also detected in late stages of spermatogenesis. During rat sexual maturation, both the testicular TAG content and the arachidonic acid content in the TAG fraction peaked at 30 d, matching the highest expression of Gpat2 mRNA and protein. These results strongly suggest that GPAT2 expression is linked to arachidonoyl-CoA incorporation into TAG in spermatogenic germ cells.

Palavras-chave : Glycerol-3-phosphate acyltransferase-2; Arachidonic acid; Triacylglycerols; Spermatogenesis.

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