Resumo

PONCE DE LEON, Patricia; LAMAGNI, Ernesto; IVANCOVICH, Juan José  e  BOTTAI, Hebe. Efecto de Trichinella spiralis y de Trichinella patagoniensis n.sp. sobre la desialización. Acta bioquím. clín. latinoam. [online]. 2019, vol.53, n.1, pp.37-42. ISSN 0325-2957.

Sialic acid has important biological functions, many of which determine its participation in the immune response. The objective of this paper was to compare the effect of Trichinella spiralis and Trichinella patagoniensis n.sp. on erythrocyte desialization. Work was performed on 10 larval concentrates of muscle larvae of T. spiralis and 10 of T. patagoniensis of the same larval concentration. The treatment was carried out incubating the sediment of fresh erythrocytes with an equal volume of larval concentrate (37 °C), taking samples at 30, 60 and 90 minutes. The controls were incubated in the same way treated with saline solution. Titration of aggregation by Polybrene Method was applied and the CexpST was determined. The results showed that the mean value of CexpST in erythrocytes with T. spiralis was significantly lower than in the globules treated with T. patagoniensis, for all the studied times. The increase in treatment time also significantly decreased the mean value of CexpST for the two species, being significantly lower at 90 minutes of incubation than at 60 minutes and these in turn lower than at 30 minutes. It is concluded that T. spiralis caused greater erythrocyte desialization than T. patagoniensis in the experimental conditions studied.

Palavras-chave : Trichinella spiralis; Trichinella patagoniensis n.sp; Desialization.

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