Resumo

PONCE DE LEON, Patricia  e  LOPEZ MURUA, Gabriel. Trichinella spiralis and Trichinella patagoniensis: study of globular charge applying Alcian Blue method. Acta bioquím. clín. latinoam. [online]. 2020, vol.54, n.4, pp.461-466. ISSN 0325-2957.

The sialic acid residues of the surface glycoproteins are mainly responsible for the erythrocyte negative charge. The objective of this work was to study alterations of globular charge produced by Trichinella spiralis and Trichinella patagoniensis. Work was carried out on muscle larvae concentrates of both species and fresh erythrocytes. The treatment was performed by incubating the globular pellet with equal volume of larval concentrate (1 and 2 hours). Controls were incubated with saline solution. The Alcian Blue method was applied and the experimental coefficient of erythrocyte anion charge (expCEAC) was determined. The results showed that the globular charge decreased with increasing treatment time for both species. The expCEAC values of the suspensions incubated with T. spiralis were lower than with T. patagoniensis, indicating that T. spiralis produced a greater decrease in charge than T. patagoniensis. It is concluded that the desialization produced by both species is not the same, suggesting that the host-parasite relationship that would be established in vivo would be different.

Palavras-chave : Trichinella spiralis; Trichinella patagoniensis; Globular charge; Alcian Blue method.

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