Resumo

CACERES, María Emilia et al. Caracterización genotípica y biofilms de E. coli shigatoxigénica aisladas de casos clínicos humanos. Acta bioquím. clín. latinoam. [online]. 2022, vol.56, n.2, pp.171-180. ISSN 0325-2957.

Shigatoxigenic Escherichia coli (STEC) is involved in the development of hemolytic uremic syndrome, among other diseases that are relevant to public health and food safety worldwide. The ability of STEC to form biofilms in food and on different surfaces could lead to cross-contamination by shedding bacterial cells. The aim of this work was to detect the presence of genes encoding adherence factors by the PCR technique and to determine the biofilm formation ability by culture in 96-well polystyrene microplates and the crystal violet technique, in STEC strains isolated from human clinical samples in Mar del Plata city, Argentina. The most frequent adherence gene profile was efa1, iha, fimCD, ehaA, lpfA1-3, lpfA2-2, cah (43.9%). All STEC strains formed biofilms with optical density values between 0.209 and 3.251. Also, the 54.4% (31/57) of STEC strains were classified as strong biofilm formers. The ability of STEC to form biofilms constitutes an evident risk in the transmission of this pathogen to humans, which must be taken into account for its surveillance and control.

Palavras-chave : Shigatoxigenic Escherichia coli; Hemolytic uremic syndrome; Biofilms; Adherence factors; Shigatoxigenic Escherichia coli.

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