Resumo

CARRION, Natalia et al. Identificación directa con MALDI-TOF desde el frasco de hemocultivo a partir de subcultivos con incubación corta. Estudio multicéntrico. Acta bioquím. clín. latinoam. [online]. 2023, vol.57, n.4, pp.8-8. ISSN 0325-2957.

The objective of this work was to evaluate the performance of the identification carried out with MALDI-TOF from the 3-5 h incubation of subcultures of monomicrobial positive blood cultures that was compared with that obtained with the 24 h incubation of the same subcultures. The Vitek-MS system (bioMérieux, France) was used in two hospitals and the Micro-Flex LT system (Bruker, Daltonics) in one. With a short incubation, MALDI-TOF correctly identified 5/5 of the yeasts, 91.1% (153/168) of the gram-positive bacteria, 96.7% (119/123) of the gram-negative bacilli and 93.6% (277/296) of the total strains. Identification by means of MALDI-TOF with a short incubation of subcultures of blood cultures in solid media is a practical, simple and reliable method.

Palavras-chave : MALDI-TOF; Blood cultures; Bacteremia.

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