Resumo

ZARATE, M. S.; SERRUTO, G.  e  SMAYEVSKY, J.. In vitro activity of tigecycline in clinical isolates of multidrug resistant Acinetobacter spp. Comparison of different methods of evaluation. Rev. argent. microbiol. [online]. 2010, vol.42, n.1, pp.53-56. ISSN 0325-7541.

Infections caused by multidrug resistant (MDRA) Acinetobacter spp. have increased worldwide. Tigecycline provides a new therapeutic option for treating these infections. We conducted a study tailored to validate an in house methodology by broth microdilution (BM) vs. commercial BM in 32 MDRA isolates. Sixty MDRA isolated in 2 time periods (2000-2003 and 2006-2008) were compared by BM, agar dilution (AD) and disk diffusion (DD) methods, as described by the CLSI and the MIC₉₀ for each period was determined. Susceptibility was interpreted by using the breakpoints suggested by the FDA for Enterobacteriaceae. The correlation between the methodologies was performed by a scattergram, and the errors between methods were calculated. The correlation coefficients between AD and BM, and BM and DD were, r: 0.68 in both cases. For 2002-2003, the MIC₉₀ for BM was: 1 g/ml, and for 2006-2008: 2 μg/ml. Using the FDA breakpoints for DD, we observed an unacceptable minor error (17.6%) because of false-intermediate values, considering the MIC₉₀ was 1-2 μg/ml.

Palavras-chave : Tigecycline; Acinetobacter spp.; Multidrug resistance.

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