Resumo

BELFIORE, Carolina; CURIA, María V  e  FARIAS, María E. Characterization of Rhodococcus sp. A5_wh isolated from a high altitude Andean lake to unravel the survival strategy under lithium stress. Rev. argent. microbiol. [online]. 2018, vol.50, n.3, pp.311-322. ISSN 0325-7541.  http://dx.doi.org/10.1016/j.ram.2017.07.005.

Lithium (Li) is widely distributed in nature and has several industrial applications. The largest reserves of Li (over 85%) are in the so-called "triangle of lithium" that includes the Salar de Atacama in Chile, Salar de Uyuni in Bolivia and Salar del Hombre Muerto in Argentina. Recently, the use of microorganisms in metal recovery such as copper has increased; however, there is little information about the recovery of lithium. The strain Rhodococcus sp. A5_wh used in this work was previously isolated from Laguna Azul. The assays revealed that this strain was able to accumulate Li (39.52% of Li/g microbial cells in 180 min) and that it was able to grow in its presence up to 1 M. In order to understand the mechanisms implicated in Li tolerance, a proteomic approach was conducted. Comparative proteomic analyses of strain A5_wh exposed and unexposed to Li reveal that 17 spots were differentially expressed. The identification of proteins was performed by MALDI-TOF/MS, and the obtained results showed that proteins involved in stress response, transcription, translations, and metabolism were expressed under Li stress. This knowledge constitutes the first proteomic approach to elucidate the strategy followed by Rhodococcus to adapt to Li.

Palavras-chave : Lithium; Extremophiles; Proteomic; Stress adaptation; Rhodococcus.

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