Resumo

PENG, Tao et al. Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3. Rev. argent. microbiol. [online]. 2019, vol.51, n.2, pp.170-178. ISSN 0325-7541.  http://dx.doi.org/10.1016/j.ram.2018.05.005.

Steroids, including testosterone, estrone, 17β-estradiol, estriol and 17β-ethinyl estradiol, are harmful not only to the population dynamics of aquatic life forms but also to public health. In this study, a marine testosterone-degrading bacterium (strain N3) was isolated from Nanao Island in the South China Sea. In addition, the strain could also use 17β-estradiol (E2), 17β-ethinyl estradiol (EE2), estriol (E3) or cholesterol as a sole carbon source. According to the 16S rRNA gene sequence analysis, strain N3 was identified as Vibrio sp. Further characterization showed that the strain is aerobic, gram-negative, and mobile and exhibits resistance to ampicillin, carbenicillin, penicillin and spectinomycin. For enhancing its capacity of testosterone degradation, the Plackett-Burman factorial design and the central composite design were used to optimize the culture condition. Under optimal conditions, 92% of testosterone was degraded by Vibrio sp. N3 in 48 h.

Palavras-chave : Steroids; Testosterone; Biodegradation; Vibrio sp. N3; Plackett-Burman design.

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