Resumo

RIVELLI ZEA, Sofía M. et al. Molecular characterization of Shiga toxin producing Escherichia coli isolated from 2 livestock establishments of Paraguay. Rev. argent. microbiol. [online]. 2020, vol.52, n.2, pp.71-80. ISSN 0325-7541.  http://dx.doi.org/10.1016/j.ram.2019.07.001.

Shiga toxin-producing Escherichia coli (STEC) is a food-borne pathogen in humans, with cattle being the main reservoir. The objective of this study was to determine the carrying of STEC in Paraguayan bovines and to analyze the virulence profile and serotypes of these isolates. A total of 197 samples of bovine fecal samples and an average of 5 to 50 colonies from stx 1 /stx 2 positive samples were studied. The stx 1 , stx 2 , saa, ehxA and eae genes were amplified by PCR. 84.8% of the cattle were carriers of STEC. The predominant virulence profiles were stx 2 and stx 2 /saa/ehxA. The serotyping was performed by agglutination reactions for 60 selected isolates, resulting in isolation of serogroup O103, which could produce infections in humans. This work shows the first data of STEC carriers in Paraguayan cattle, and indicates the need for other studies with greater territorial coverage for a complete vision of this phenomenon.

Palavras-chave : Shiga toxin-producing Escherichia coli; Cattle; Paraguay.

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