Resumo

BABOT, Jaime Daniel; ARGANARAZ-MARTINEZ, Eloy; APELLA, María Cristina  e  CHAIA, Adriana Perez. Rapid Identification of new isolates of Acidipropionibacterium acidipropionici by fluorescence in situ hybridization (FISH). Rev. argent. microbiol. [online]. 2022, vol.54, n.4, pp.51-60.  Epub 01-Dez-2022. ISSN 0325-7541.  http://dx.doi.org/10.1016/j.ram.2022.02.006.

Acidipropionibacterium acidipropionici is widely used for many applications, suchas propionic acid production, cereal silage, and also as probiotic. Due to this plethora of appli-cations, new isolates of A. acidipropionici with improved features are being searched for. Thesenew isolates must be accurately identified, however, most approaches become expensive andtime-consuming when the number of isolates is high. On the contrary, fluorescence in situhybridization allows the affordable, reliable, and rapid identification of microorganisms in purecultures and environmental and medical samples. Therefore, the aim of this work was to applya fluorescent in situ hybridization probe for the reliable identification of new A. acidipropioniciisolates. To this end, probe Pap446, specific for A. acidipropionici, was validated by hybridiza-tion assays with strains of this species from different origins, other species of the same genusor family, and unrelated genera. Eight isolates with propionibacterium characteristics wereobtained from milk and feces of cows. Probe Pap446, hybridized only with isolates III and VI.The identity of these isolates was further confirmed by PCR using group and species-specificprimers for propionibacteria and 16S rDNA sequencing.

Palavras-chave : Acidipropionibac teriumacidipropionici; Fluorescence in situhybridization; Identification ofbacteria.

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