Resumo

ALOISI, P.V  e  RUPPEL, S. Propiedades bioactivas y nutricionales del polen apícola de la provincia del Chubut, Argentina. RIA. Rev. investig. agropecu. [online]. 2014, vol.40, n.3, pp.296-302. ISSN 1669-2314.

Pollen collected by bees is a beekeeping product used in human diet for its high nutritional value and for being a balanced food. It consists in a mixture of pollen with different botanical origin, nectar and bee secretions. This beehive product rich in sugars, lipids and polyphenolic compounds, mainly flavonoids, has also bioactive properties such as pharmacological and antioxidant. The aim of this study was to determine the nutritional composition and antioxidant activity of bee pollen from the most important apicultural areas from Chubut: the Andean region and the region of the lower valley of the Chubut River (VIRCH). Bradford method was used for the determination of protein content and the values were expressed in % (g protein/100 g dry pollen). The amounts of total polyphenols and flavonoids were determined by the Folin-Ciocalteu method and the spectrophotometric technique of aluminum nitrate (Al (NO3)3) respectively. The antioxidant activity was evaluated using the technique of free radical 1, 1 -diphenyl -2 - picrylhydrazyl (DPPH *). The protein content presented a mean value of 20.2%. The content of total polyphenols and flavonoids from both apicultural regions varied between 50.5 and 163.9 mg of gallic acid (GAE) / g of dry pollen and 10.3 and 65.6 mg of rutin (RE)/ g of dry pollen. The antiradical activity in pollen extracts from both regions was high and the EC50 ranged from 0.34 mg / ml and 5.82 mg / ml. The highest antioxidant capacity corresponded to bee pollen from VIRCH, which also exhibited a higher content of phenolic compounds.

Palavras-chave : Antioxidants; Bradford; Proteins; Argentinean Patagonia.

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