Resumo

FLORES, C. et al. La administración de selenio disminuye la lipoperoxidación en semen de toros Brahman. Rev. vet. [online]. 2014, vol.25, n.2, pp.95-99. ISSN 1669-6840.

Lipid peroxidation, one of the effects of oxidative stress, is associated with impaired sperm motility and viability because of biochemical and structural alterations of the plasma membrane which causes infertility. Of interest is the study of therapeutic alternatives for enhancing the reproductive condition of the bulls. In this study the antioxidant action of selenium and its effect on semen quality was determined. Healthy grazing Brahman bulls with 15-18 months of age, divided into two groups, were used for the trial. The experimental group received an intramuscular dose of 0.22 mg/20 kg liveweight /day, during 5 days. The sample was taken with electroejaculator at day zero, 15 and 30 post-treatment. Diene conjugates (DC, extracted with isopropanol) and malondialdehyde (MDA TBARS) were measured. Semen quality (motile sperm, percentage of progressive motility, morphology, sperm vitality and presence of acrosomes) and indicators of lipid peroxidation were evaluated. Data were analyzed by ANOVA (p=0.05). In the study of semen, quality groups did not differ significantly. Compared to the control group, selenium-treated animals showed no significant differences in DC at 15 and 30 days. On the other hand, MDA revealed significant differences at 30 days post treatment, showing lower values (1.09 ±0.1 nmol/mg protein) compared to control group (1.58 ±0.2 nmoles/mg protein). In conclusion, seminal selenium decreases lipid peroxidation and preserves semen quality.

Palavras-chave : Brahman bull; lipid peroxidation; infertility; selenium; seminal quality.

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