Resumo

SLANAC, A.L.; SGROPPO, S.C.; KUCSEVA, C.D.  e  BALBUENA, O.. Caracterización electroforética de proteínas de la "paja amarilla" (Sorghastrum setosum) incubada en rumen de bovinos. Rev. vet. [online]. 2014, vol.25, n.2, pp.120-125. ISSN 1669-6840.

With the objective of characterize the protein fractions of Sorghastrum setosum ("sandysoil indiangrass") during its ruminal degradation, 3 g of acetone powder obtained from samples subjected to different ruminal incubation times were taken (0; 6; 12; 24; 48; 72 and 120 hours). Proteins present in the acetone residue were solubilized in Tris buffer pH 8 and quantitated by the Lowry method. The characterization was carried out by denaturing polyacrylamide gel electrophoresis (12% containing SDS) according to the modified Laemmli buffer system (1970). The qualitative analysis performed at zero hour showed intense bands of 87; 64; 58; 55; 51; 47; 46; 44; 42; 27 and 14 kDa. For the protein profiles, intense bands of 46; 42 and 14 kDa were detected, as well as others of lower intensity of 64; 58 and 51 and finally a group of high molecular weight in the region of 87 kDa. During the degradation process, modifications of the protein profile were observed, being detected new bands of high and half PM, as well as the appearance and increase of low PM bands. During the process of ruminal degradation it was observed that the bands of 24, 14 and 55 kDa were present during the 120 hours of study; this prolonged permanency indicates the scarce ruminal degradability of this gramineous.

Palavras-chave : bovino; rumen; proteína; electroforesis; Sorghastrum setosum.

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