Resumo

RAMOS VEINTIMILLA, R. A. et al. In vitro propagation of tree species of the Paulownia genus under the conventional propagation system. Quebracho (Santiago del Estero) [online]. 2017, vol.25, n.2, pp.69-79. ISSN 0328-0543.

This study aimed to evaluate an in vitro propagation method from buds of Paulownia elongata, P. fortunei, and the hybrid (Paulownia fortunei x elongata) through direct organogenesis in order to facilitate the establishment, propagation, rooting, and acclimatization of Paulownia’s seedlings for large-scale production purposes. Four phases were executed: (1) introduction and in vitro disinfection with three procedures and two culture medium types, (2) in vitro propagation with four culture media for multiplication, (3) in vitro rooting with three semisolid culture media based on different doses of auxins and citochinines, and (4) acclimatization in which the response to 2 substrates under greenhouse conditions were evaluated. The most effective disinfection was achieved with the protocol involving fungicides/bactericides + PVP + hypochlorite sodium + ascorbic acid + tween 20. The highest propagation index (17 to 20 nodal segments) was achieved with the three hormones doses investigated; however, the M&S culture type supplemented by 0.05 mg L-1 of ANA and 2 mg L-1 of BAP, showed just 15 nodal segments. The most effective rooting method was M&S with 0.4 mg L-1 IBA. The substrate composed of pomina and black soil (1:1) allowed 100 % of seedling survival and the highest plant height. In conclusion, in vitro propagation under the conventional system could be used for massive multiplication of these forest species.

Palavras-chave : Asexual propagation; Phytohormones; Culture media; Explants; Disinfection; Seedling acclimatization.

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