Resumo

ALVARENGA, Adriana E; AREVALO, María I; CASTRO, Guillermo R  e  ABATE, Carlos M. Selection and Characterization of α-L-Rhamnosidase Activity Producers of Psychrotolerants Strains. Rev. cienc. tecnol. [online]. 2015, n.24, pp.48-54. ISSN 1851-7587.

α-L-rhamnosidase plays a key role in grape juice and derived beverages processing by enhancing the aroma and in citric juices by removing bitterness. The aim of this work was to study and partially characterize marine microorganisms from different coastal areas of the Beagle Channel and intestinal contents of benthic organisms with α-L-rhamnosidase activity. A total of 10 microorganisms were selected because of their proper growth in MMB supplemented with rhamnose as the sole carbon source, at 8 and 20 °C. According to the quantitative determination of the α-L-rhamnosidase activity, G33 isolate showed the highest activity values at 37 °C (411.7 U.L-1), followed by F6 isolate at 30 °C (250.2 U.L-1). Molecular identification methods classified the isolates F6 and G33 as Serratia and Pseudomonas genera respectively. The results showed that α-L-rhamnosidase enzymes from Serratia sp. F6 and Pseudomonas sp. G33 strains are promising to be used in biotechnological strategies.

Palavras-chave : α-L-rhamnosidase; Pseudomonas sp. G33; Serratia sp. F6; Beagle Channel.

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