Resumo

ARENCIBIA-ARREBOLA, Daniel Francisco; ROSARIO-FERNANDEZ, Luis Alfredo  e  SUAREZ-FERNANDEZ, Yolanda Emilia. Sensibilidad de distintas líneas murinas a la ciclofosfamida medida a través del ensayo de micronúcleos en células de la medula ósea. BAG, J. basic appl. genet. [online]. 2011, vol.22, n.2, pp.25-32. ISSN 1852-6233.

The aim of this study was to evaluate the sensibility of different murine lines to the cyclophosphamide (CF) measure through the micronucleus assay in bone marrow cells. Being used mice of both sexes of the Balb/c, NMRI, OF-1 and C57BL/6/cenp lines. The use of the best line was suggested in this assay on the base of significant appearance of low indexes spontaneous and high induced indexes to the CF. It was obtained as a result that the Balb/c line in both sexes differs significantly of the other evaluated lines. In this line the lowest results in cytotoxicity index (relationship among young erythrocytes/mature erythrocytes) and genotoxicity indexes (erythrocytes percent in bone marrow with micronucleus) were observed, at the same time of responding from an acceptable form to the CF. Also the line C57/BL6/cenp was the less efficient and more sensitive to the mutagen, being obtained the highest spontaneous and induced results. These results allow to suggest that the Balb/c mice, is the most appropriate line to use as biomodel in the micronucleus assay, when it is used the CF as positive control.

Palavras-chave : Micronucleus assay; Mice; Bone marrow; Cyclophosphamide.

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