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BAG. Journal of basic and applied genetics
versão On-line ISSN 1852-6233
Resumo
MENONE, M.L; PEREZ, D.J; LUKASZEWICZ, G e CAMADRO, E.L. Identification of freshwater hydrophytes for genotoxicity assessment of aquatic pollutants. BAG, J. basic appl. genet. [online]. 2015, vol.26, n.1, pp.9-17. ISSN 1852-6233.
For exploring the potential use of hydrophytes in genotoxicity bioassays for aquatic contaminants, as a complement or replacement of the traditional and standardized use of terrestrial macrophytes (Allium cepa (L.), Pisum sativum (L.), Tradescantia sp. (L.), among others), a preliminary study was carried out in an attempt to select at least one wild wetland taxonomic species that had to meet the following requirements: to have a low basic chromosome number and a high mitotic index (MI), and to be easily propagated in the laboratory. Three field vegetation surveys were carried out in La Brava Lake (Buenos Aires province, Argentina). Seeds of the most representative species were collected and placed in Petri dishes for germination. Cytogenetic studies were, then, performed in root tips. Two of the species, Bidens laevis (L.) and Solanum chenopodioides (Lam.) fulfilled the requirements for their use in the analysis of genotoxicity of aquatic contaminants.
Palavras-chave : Native aquatic macrophytes; Basic chromosome number; Mitotic index; Cytogenetic bioassays; Genotoxicity.