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Phyton (Buenos Aires)

versão On-line ISSN 1851-5657

Resumo

OCHOA FUENTES, YM et al. Evaluation in vitro of the anti-fungal activity of four methanol plant extracts for the control of three species of Fusarium spp. Phyton (B. Aires) [online]. 2012, vol.81, n.1, pp.69-73. ISSN 1851-5657.

We evaluated the in vitro antifungal activity of extracts of peruvian peppertree (Shinu smolle), cherimoya (Annona cherimola), cinnamon (Cinnamomum zeylanicum) and tobacco (Nicotiana glauca) on the mycelial growth and sporulation of Fusarium oxysporum, Fusarium culmorum and Fusarium solani. The research was conducted at the Universidad Autónoma de Aguascalientes (UAA) and the Universidad Autónoma Agraria Antonio Narro (UAAAN). The species of Fusarium were previously identified in the UAA Laboratory of Parasitology. The extracts were obtained in the laboratory of Toxicology of the UAAAN. The methodology of poisoned culture medium was used to determine the (1) inhibition of mycelial growth, (2) average effective dose (ED50) and (3) number of conidia at various concentrations of the extracts. The cherimoya and cinnamon extracts exhibited inhibitory effects on mycelial growth and sporulation. In contrast, tobacco and Peruvian peppertree extracts did not show any inhibitory effect. However, the first of them increased conidia production when extract concentration was increased. In relation to ED50, cinnamon extracts were effective to control Fusarium species in doses of 330 to 539 ppm. On the other hand, the best control of the cherimoya extract was at 593 ppm against F. culmorum, while higher doses from 2060 to 2571 ppm were required to control F. oxysporum and F. solani, respectively. We conclude that cinnamon and cherimoya extracts showed inhibitory effects on the mycelial growth and sporulation of F. oxysporum, F. culmorum and F. solani.

Palavras-chave : Fusarium oxysporum; Fusarium culmurorum; Fusarium solani; Mexico.

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