Abstract

CORTEZ, J. V.; SEGURA, G. T.  and  MURGA, N. L.. Impact of cell reprogramming (fibroblast cells and clusters) on the production of bovine clones. Rev. vet. [online]. 2021, vol.32, n.2, pp.216-220. ISSN 1669-6840.  http://dx.doi.org/10.30972/vet.3225746.

18.

Conventional cloning consists of several processes that have a direct effect on embryonic production and the pregnancy rate, a primary factor is the reprogramming of the cell used as a nucleus donor. The objective of the present study was to evaluate the impact of cell reprogramming (fibroblast cells and clusters) on the production of bovine clones. The nucleus donor cells were obtained from fibroblasts and cumulus cells for their reconstruction with ovules and cultured for 7 d until the blastocyst incubation / hatching phase, to be subsequently transferred to recipients and evaluated on day 28, 60 and 75 of gestation. The number and percentage of reconstructed cytoplasts were evaluated at the time of fusion, obtaining greater efficiency with the cumulus cells (51,7 ± 7,8) (p<0,05). The percentage of embryos obtained on day 7 was higher with fibroblast cells (36,1 ± 8,4) compared to cumulus cells (34,1 ± 10,0). At 28, 60 and 75 days post transfer, pregnancy permanence was evaluated, being significantly higher (p<0,05) the embryos generated with cumulus 16 cells (12,0%). The efficiency of cell reprogramming is similar in the origin of the cell, however there is a higher percentage of pregnancy retention with cumulus cells as nucleus donor.

Keywords : assisted reproduction; cleavage; reconstruction; cell / ovule complex.

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